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2011, Number 2

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Cir Gen 2011; 33 (2)

Heparin in a deep second degree burn in a murine model

Sigler L, Krotzsch E, Bretón-Mora F, Padilla L, DiSilvio M, Álvarez-Jiménez R

Language: Spanish
References: 23
Page: 86-90
PDF size: 122.21 Kb.


ABSTRACT

Objective: To demonstrate the effects of heparin topically or intraperitoneally applied on the recovery from second degree burns in a murine model.
Setting: Third level health care center Centro Médico Nacional “20 de Noviembre”, ISSSTE.
Design: Experimental study.
Statistical analysis: t de Student.
Material and methods: We studied three groups of five rats each in which a second-degree thermal burn was produced on their back, 10% of the body surface. The control group received 5 ml of saline solution on the burned area per day during 30 days; the second group received heparin and the third group received intraperitoneally applied heparin and saline solution applied topically on the burn. A biopsy of the burned area was obtained, alkaline phosphatase was determined, and photographs were taken at days 0, 15, and 30 after causing the burn. Biopsy demonstrated a similar dermal burn degree in the three groups.
Results: When rats received heparin, either topically or peritoneally, they had a faster healing (50% faster) and with less scars than the control group. Level of alkaline phosphatase revealed a higher inflammatory response in the control group. All differences were significant at P ‹ 0.05.
Conclusions: Heparin, administered either in the peritoneum or topically in similar groups of rats with second degree burns, induced wound healing in half the time with less inflammation, and better epithelial recovery than treatment with saline solution without heparin.


REFERENCES

  1. Alrich EM. The effect of heparin on the circulating blood plasma and proteins in experimental burns. Surgery 1949; 25: 676-680.

  2. McCleery RS, Schaffarzick WH, Light RA. An experimental study of the effect of heparin on local pathology of burns. Surgery 1949; 26: 548-564.

  3. Elrod PD, McCleery RS, Ball CO. An experimental study of the effect of heparin on survival time following lethal burns. Surg Gyn Obstet 1951; 92: 35-42.

  4. Dolowitz DA, Dougherty TF. The use of heparin as an anti-inflammatory agent. Laryngoscope 1960; 70: 873-884.

  5. Saliba MJ Jr. Heparin in the treatment of burns. JAMA 1967; 200: 650-651.

  6. Saliba MJ Jr,. Griner LA. Heparin efficacy in burns. I Significant early modifications of experimental third degree guinea pig thermal burn and ancillary findings. A double blind study. Aerosp Med 1970; 41: 179-87.

  7. Saliba MJ Jr. Heparin efficacy in burns. II. Human thermal burn treatment with large doses of topical and parenteral heparin. Aerosp Med 1970; 41: 1302-1306.

  8. Saliba MJ Jr, Dempsey WC, Kruggel JL. Large burns in humans. Treatment with heparin. JAMA 1973; 225: 261-269.

  9. Saliba MJ Jr, Saliba RJ. Heparin in burns. Dose related and dose dependent effects. Thromb Diath Haemorrh 1975; 33: 113-23.

  10. Carr J. The anti-inflammatory action of heparin: heparin as an antagonist to histamine, bradykinin, and prostaglandin E1. Thromb Res 1979; 16: 507-516.

  11. Ehrlich HP, Griswold TR, Rajaratnam JB. Studies on vascular smooth muscle cells and dermal fibroblasts in collagen matrices. Effects of heparin. Exp Cell Res 1986; 164: 154-162.

  12. Saliba MJ Jr. The effects and uses of heparin in the care of burns that improves treatment and enhances the quality of life. Acta Chir Plast 1997; 39: 13-16.

  13. Cribbs RK, Luquette MH, Besner GE. Acceleration of partial-thickness burn wound healing with topical application of heparin-binding EGF-like growth factor (HB-EGF). J Burn Care Rehabil 1998; 19: 95-101.

  14. Cen Y, Luo P, Liu XX. The effect of heparin on wound healing of second-degree burned rats. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2000; 14: 264-267.

  15. Reyes AE, Aztiazaran JA, Chávez CC, Jaramillo F, Saliba MJ Jr. Burns treated with and without heparin: Controlled use in a thermal disaster. Ann Burns Fire Disasters 2001; 14: 183-191.

  16. Saliba MJ Jr. Heparin in the treatment of burns: a review. Burns 2001; 27: 349-358.

  17. Matzsch T, Bergqvist D, Blomquist P, Jiborn H. Influence of standard heparin or low molecular weight heparin on healing of abdominal wounds and colonic anastomoses in rats. Acta Chir Scand 1987; 153: 593-598.

  18. Galvan L. Effects of heparin on wound healing. J Wound Ostomy Continence Nurs 1996; 23: 224-226.

  19. Ehlrich HR, Griswold TR, Rajaratanam JBM. Studies on vascular smooth muscle cells and dermal fibroblasts in collagen matrices. Effects of heparin. Exp Cell Res 1986; 164: 154-62.

  20. Suárez A, Salgado RM, Apis Z, Krötzsch E. Inducción del tejido de granulación por pasta de Lassar vs colágena-polivinilpirrolidona en úlceras por insuficiencia venosa. Cirugía Plástica 2004; 141: 5-13.

  21. Young E. The anti-inflammatory effects of heparin and related compounds. Thromb Res 2008; 122: 743-752.

  22. Barretto MG, Costa Mda G, Serra MC, Afiune JB, Praxedes HE, Pagani E. Comparative study of conventional and topical heparin treatments for burns analgesia. Rev Assoc Med Bras 2010; 56: 51-55.

  23. Toon MH, Maybauer MO, Greenwood JE, Maybauer DM, Fraser JF. Management of acute smoke inhalation injury. Crit Care Resusc 2010; 12: 53-61.




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