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2015, Number 1

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Rev Cubana Invest Bioméd 2015; 34 (1)

Oxidative effect of severe iron deficiency anemia in male justweaned Wistar rats

García HY, Morejón CA, Bourg LV

Language: Spanish
References: 21
Page: 44-53
PDF size: 208.43 Kb.


ABSTRACT

Introduction: the physiological process leading to nutritional iron deficiency anemia involves the depletion of mineral deposits, a decrease in the supply of iron for erythrocyte synthesis, and finally a reduction in the concentration of blood hemoglobin. Some studies have shown increased tissue susceptibility to oxidative damage in iron deficiency anemia, while others do not report any difference between healthy and anemic persons.
Objective: Determine the oxidative effect of severe iron deficiency anemia in a rat experimental model.
Methods: male just-weaned Wistar rats were fed for 45 days a purified diet based on casein as a source of proteins. Two groups were formed, each with seven animals. One group received an iron deficient diet (18 mg/kg) and the other a diet with normal iron content (42 mg/kg).
Results: in the control group receiving a diet with normal iron content, oxidative damage to lipids and proteins was greater at the duodenal mucosa, the area where the intestinal absorption of iron takes place. In anemic animals, oxidative damage was greater in the liver (p ‹ 0.05). Increased oxidative damage to the liver in anemic animals is explained by the movement of mineral from this organ to erythroid tissue to make up for the reduction in intestinal absorption of the mineral caused by the iron deficient diet. The study shows one more of the adverse effects of severe iron deficiency anemia.
Conclusions: oxidative damage in the liver is an adverse side effect of severe iron deficiency anemia in rats.


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