Figueroa-García MC, Rivera-Valencia M, Sosa-Durán EE, Saavedra-Molina FA, Mejía-Zepeda R

Language: Spanish
References: 19
Page: 23-30
PDF size: 153.16 Kb.

ABSTRACT

The fasting is a factor that modifies glycaemia, which is regulated by the supply and the kind of carbohydrates in the diet, the lasting of the ingestion, the absorption time, as well as on the insulin and glucagon secretion, and its coordinated effects on the glucose metabolism. In the other hand, the glycaemia is also affected by factors such as: age, physiological state, illness-health processes, nutritional condition, and stressing environmental factors. It is important to indicate the appropriate fasting time for the determination of the blood glucose concentration (BGC) in these periods, minimizing the stress from long fasting, especially in diabetic subjects. In the present study, it was determined the required fasting time for evaluation of blood glucose in male rats with type 2 diabetes. For these purposes, 48-h-old male Wistar rats were administered i.p. with streptozotocin (STZ) at 125 mg/kg of body weight. In order to know the metabolic alterations, the concentrations of glucose, cholesterol, and triglycerides were measured in blood. Every 30 days the glycemic profile during fasting (GPF) was done and then after the glucose tolerance curve (GTC). The obtained results shown that the glycemic control tend to stabilize as long as the age of normoglycemic rats increase reaching basal concentrations (4.4 mM) at 4 h postprandrio. However, in diabetic animals the control of glycaemia varies according with the period of life, in such a way that during infant and puberty, the concentrations of basal glycaemia (4.4 and 6 mM, respectively) are obtained with a fasting of 4 h, whereas in adult animals there is a negative correlation between the BGC and the time, reaching sometimes values ‹ 4.0 mM. The literature points out an 8 h fasting for evaluation of glycaemia in healthy males, but the results obtained in our work shows that in type 2 diabetic rats, the best moment for measurement of this parameter is at 4 h.

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