2016, Number 3
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Rev Cubana Plant Med 2016; 21 (3)
Use of ultrasound for extraction of the apolar fraction in leaves of Mangifera indica L. (mango tree)
Acosta EJ, Salomón IS, Sevilla FI, Nuevas PL
Language: Spanish
References: 13
Page: 261-271
PDF size: 274.60 Kb.
ABSTRACT
Introduction: mangiferin is a natural glucosyl xanthone found in several parts of
Mangifera indica L. (mango tree) which has a great variety of pharmacological effects.
Its isolation from leaves is of great importance, considering the pharmacological
properties attributed to it. A national procedure has been developed in Cuba to obtain
mangiferin from
M. indica leaves using a traditional stirred tank procedure which
includes a degreasing stage. However, studies continue to be conducted about the
use of unconventional extraction techniques such as ultrasound, due to the speed and
efficacy with which phytoconstituents may be obtained.
Objective: evaluate the apolar fraction extraction stage of the process of ultrasound
extraction of mangiferin from M. indica leaves.
Methods: M. indica leaves were ground and stored in plastic bags at ambient
temperature and humidity. Operating parameters (time and solvent/plant material
ratio) were evaluated at the degreasing stage with a response surface factorial
design, using ultrasound.
Results: the best experimental value (29.03 ± 1.10 mg/g) is obtained for a time of
90 min and a solvent volume/plant material ratio of 47 ml/g, using n-hexane as
solvent.
Conclusions: the ultrasound technique is appropriate for apolar fraction extraction of
mangiferin from
M. indica leaves.
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