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TIP Revista Especializada en Ciencias Químico-Biológicas

2019, Number 1

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TIP Rev Esp Cienc Quim Biol 2019; 22 (1)

Nontuberculous mycobacteria from mexican archaeological sites

Alcalde-Vázquez R, González-y-Merchand JA, Medina-Jaritz NB, Olvera-Ramírez R

Language: English
References: 23
Page: 1-9
PDF size: 447.71 Kb.


ABSTRACT

We examined several buildings of nine archaeological sites in Mexico for the presence of mycobacteria and we could isolate forty-five nontuberculous mycobacteria (NTM). These were isolated from biofilms using selective media containing different antibiotics and dyes. Identification of the isolated mycobacteria was carried out, first, by a molecular identification by means of a mycobacteria-specific PCR using bacterial lysates of the acid-fast bacilli followed by species identification by comparing of three molecular markers: genes rrs (16SrRNA), hsp65 and rpoB. Furthermore, the physiographic data of the archaeological zones under study was related to the number of acid-fast microorganisms using a univariate analysis of variance. From the 45 isolated mycobacteria, 21 were Mycobacteroides chelonae; seven, Mycobacteroides abscessus; five, Mycolicibacterium flavescens; four, Mycobacterium alvei; two, Mycobacterium fortuitum; and six, Mycobacterium sp. Most NTM were isolated from two archaeological sites: 25 from Guachimontones (Jalisco), and 13 from Atetelco (Estado de México). The statistical analysis showed that environmental factors such as climate and the temperature-humidity-precipitation interaction had the greatest influence on the presence of NTM in these archaeological zones.


REFERENCES

  1. Adékambi, T., Colson, P. & Drancourt, M. (2003). rpoB-Based Identification of Nonpigmented and Late-Pigmenting Rapidly Growing Mycobacteria. J. Clin. Microbiol., 41(12), 5699-5708.DOI: 10.1128/JCM.41.12.5699- 5708.2003

  2. Ascaso, C., Wierzchos, J., Souza-Egipsy, V. De los Rios, A. & Delgado Rodrigues, J. (2002). In situ evaluation of the biodeteriorating action of microorganisms and the effects of 103 biocides on carbonate rock of the Jeronimos Monastery (Lisbon). Int. Biodeter. Biodegr., 49, 1-12.DOI:10.1016/S0964-8305(01)00097-X

  3. Cobos-Marín, L., Montes-Vargas, J., Rivera-Gutiérrez, S., Licea-Navarro, A., González-y-Merchand J. A. & Estrada-García, I. (2003). A novel multiplex-PCR for the rapid identification of Mycobacterium bovis in clinical isolates of both veterinary and human origin. Epidemiol. Infect., 130, 485-490.DOI: 10.1017/ S095026880300829X

  4. Falkinham, J. O. (2009a). Surrounded by mycobacteria: nontuberculous mycobacteria in the human environment. J. of App. Microbiol., 107, 356-367.DOI: 10.1111/j.1365-2672.2009.04161.x

  5. Falkinham, J. O. (2009b).The Biology of environmental mycobacteria. Environ. Microbiol. Rep., 1(6), 477- 487.DOI: 10.1111/j.1758-2229.2009.00054.x

  6. Falkinham, J. O. (2015). Environmental sources of nontuberculous mycobacteria. Clin. Chest. Med., 36, 35-41.DOI: 10.1016/j.ccm.2014.10.003

  7. Garrett, T.R., Bhakoo, M. & Zhang, Z. (2008). Bacterial adhesion and biofilms on surfaces. Prog. Nat. Sci., 18, 1049- 1056. DOI: 10.1016/j.pnsc.2008.04.001

  8. González-y-Merchand, J. A., Colston, M. J. & Cox, R. A. (1996). The rRNA operon of Mycobacterium smegmatis and Mycobacterium tuberculosis comparison of promoter elements and of neighbouring upstream genes. Microbiol., 142, 667-674. DOI: 10.1099/13500872-142-3-667

  9. Gupta, R. S., Lo, B. & Son, J. (2018). Phylogenomics and comparative genomic studies robustly support division of the genus Mycobacterium into an emended genus Mycobacterium and four novel genera. Front. Microbiol., 9, 1-41.DOI:10.3389/fmicb.2018.00067

  10. Johnson, M. M. & Odell, J. A. (2014). Nontuberculous mycobacterial pulmonary infections. J. Thorac. Dis., 6(3), 210–220. DOI: 10.3978/j.issn.2072- 1439.2013.12.24

  11. Kieser, K. J. & Rubin, E. J. (2014). How sisters grow apart: mycobacterial grow and division. Nat. Rev. Microbiol., 12, 550-562. DOI: 10.1038/nrmicro3299

  12. Kirschner, R. A., Parker, B. C. & Falkinham, J. O. (1999). Humic and fulvic acids stimulate the growth of Mycobacterium avium. FEMS Microbiol. Ecol., 30, 327-332.DOI:10.1111/j.1574-6941.1999.tb00660.x

  13. Kusumi, A., Shu-Li, X., & Katayama, Y. (2011). Mycobacteria isolated from Angkor monument sandstones grow chemolithoautotrophically by oxidizing elemental sulfur. Front in Microbiol., 2, 1-7.DOI: 10.3389/ fmicb.2011.00104

  14. Manzoor, S. E., Lamber, P. A., Griffiths, P. A., Gill, M. J. & Fraise, A. P. (1999). Reduced glutaraldehyde susceptibility in Mycobacterium chelonae associated with altered cell wall polysaccharides. J. Antimicrob. Chemother., 43,759-765. DOI:10.1093/jac/43.6.759

  15. McNamara, C. J., Perry IV, T. D., Bearce, K. A., Hernández- Duque, G. & Mitchell, R. (2006). Epilithic and Endolithic Bacterial Communities in Limestone from a Maya Archaeological Site. Microb. Ecol., 51(1), 51- 64. DOI: 10.1007/s00248-005-0200-5.

  16. Pavlik, I., Kazda, J. & Falkinham, IIIJ.O. (2010). Environments Providing Favourable Conditions for the Multiplication and Transmission of Mycobacteria. En J. Kazda et al. (eds.), The Ecology of Mycobacteria: Impact on Animal’s and Human’s Health, (pp. 89-97) New York: Springer. DOI: 10.1007/978-1-4020-9413- 2 5

  17. PRASITE, 2018, Identification of Mycobacteria, http://app. chuv.ch/prasite.

  18. Romaní, A. M., Found K., Artiagas J., Schwartz T., Sabater S. & Obst U. (2008). Relevance of polymeric matrix enzymes during biofilm formation. Microb. Ecol., 56, 427-436.DOI: 10.1007/s00248-007-9361-8

  19. Sareen, M. & Khuller, G. K. (1990). Cell wall and membrane changes associated with ethambutol resistance in Mycobacterium tuberculosis H37Ra. Antimicrob. Agents Chemother, 34(9), 1773-1776. DOI: 10.1128/ AAC.34.9.1773

  20. Telenti, A., Marchesi F., Balz, M., Bally, F., Bottger E. C. & Bodmer, T. (1993). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J. Clin. Microbiol., 31, 175-178.DOI: 0095-1137/93/020175

  21. Thorel, M.F., Falkinham, J. O. & Moreau, R. G. (2004). Environmental mycobacteria from alpine and subalpine habitats. FEMS Microbiol. Ecol, 49, 343- 347. DOI:10.1016/j.femsec.2004.04.016

  22. Videla, H. A., Guiamet, P. S. & Gómez de Saravia, S. (2000). Biodeterioration of Mayan archaeological sites in the Yucatan Peninsula, Mexico. Int. Biodeter. Biodegr., 46(4), 335-341. DOI: 10.1016/S0964-8305(00)00106-2

  23. Yang, L., Sinha, T., Carlson, T. K., Keiser, T. L., Torrelles, J. B. & Schlesinger, L. S. (2013). Changes in the mayor cell envelope components of Mycobacterium tuberculosis during in vitro grow. Glycobiology, 23(8), 926-934. DOI: 10.1093/glycob/cwt029




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