2025, Number 2
CytoVision vs conventional scoring, an innovation for the dicentric assay
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Language: English
References: 30
Page: 99-107
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ABSTRACT
The dicentric assay is the gold standard of biological dosimetry to assess ionization radiation dose from any person. The objective of this work is to present a comparison between conventional dicentric scoring and semiautomatic scoring using a CytoVision analyser. Materials and methods: Human lymphocytes were irradiated with four doses of gamma rays and cultured to metaphase; cells were scored for dicentric aberrations conventionally ‘by-eye’ and with a semiautomatic CytoVision analyser. Doses estimated according to Z-score were classified as satisfactory, and dicentric frequencies obtained by the conventional and semiautomated systems were compared with the R program. Results: The physical and the estimated doses derived from our own dicentric dose response curve agreed well, the largest discrepancy was 15%. Conclusion: The study established that conventional dicentric scoring and semiautomatic scoring using a CytoVision analyser provided similar results, but the semiautomatic scoring significantly reduced the scoring time.REFERENCES
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