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2016, Number 3

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Rev Cubana Plant Med 2016; 21 (3)

Evaluation of the antiproliferative activity of methanolic extracts of plants from the Leguminosae family

Olmedo ADA, Marrone PNS, Espinosa RAF, Guerra TCP, Prashad GM

Language: Spanish
References: 24
Page: 272-283
PDF size: 147.53 Kb.


ABSTRACT

Introduction: the Leguminosae family is rich in isoflavonoids potentially effective against cancer. No studies have been conducted of Panamanian species from this family to explore their possible use in the treatment of breast and prostate cancer, two main causes of mortality among the adult population in Panama.
Objective: evaluate the antiproliferative effect of methanolic extracts from the Leguminosae family against estrogen-dependent breast cancer (ER+) and androgendependent prostate cancer (AR+).
Materials: all 69 species of plants from this family were collected from the provinces of Veraguas and Darién in the Republic of Panama. Dried, pulverized material from various parts of the plants was macerated in methanol for 24 h with stirring. The material obtained was filtered, concentrated in a rotary evaporator at ‹ 40°C, and lyophilized. The crude extracts obtained were evaluated for breast cancer cell lines MCF-7(ER+) and MCF-7(ER-), and prostate cancer cell lines (CaP (AR+)), using the antiproliferative assay with sulphorhodamine (SRB) and tetrazolium (MTT).
Results: the most active extracts against estrogen-dependent breast cancer (ER+) were obtained from Neptunia pubescens (fruit), Zygia latifolia (stem) and Albizia adinocephala (bark) with GI50 values of 1.1 to 5.9 µg/ml, whereas the most active extracts against androgen-dependent prostate cancer (AR+) were obtained from Zygia latifolia (leaves), Pithecellobium dulce (leaves) and Acosmium panamense (stem), with GI50 values of 1.2 to 5.1 µg/ml. Additionally, extract from Albizia adinocephala (bark) was active against breast cancer expressing receptor HER-2, with an CI50 of 4.8 µg/ml.
Conclusions: crude extracts from promising species could be useful as potential sources of substances with antiproliferative activity against receptor-dependent cancers (ER+), (AR+) and (HER-2+).


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