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ISSN 8541-3697 (Print)

2017, Number 1

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AbanicoVet 2017; 7 (1)

Evaluation of the antibacterial effect of ozonized olive oil against Listeria monocytogenes

Godínez-Oviedo A, Zamora-Rodríguez Z, Martínez-Juárez V, Fleitas-González E, Hernández-Rosado A, Peña-Jiménez F

Language: Spanish
References: 32
Page: 36-43
PDF size: 323.90 Kb.


ABSTRACT

The vegetable ozonized oils are a new alternative to treat humans and animals against pathogens, due to their antimicrobial capacity. The aim of this study was to determine the antimicrobial effect of olive ozonized oil against Listeria monocytogenes. Three different ozonized oils were used: T1: olive ozonized oil stored for 6 months at 4°C, T2: olive ozonized oil produced in the moment of the test, and a commercial ozonized oil (Oleozon ®). The peroxide index of the oils was determined using a volumetric method. Also, the antimicrobial activity was evaluated using a diffusion method with different concentrations of the inocula. The peroxide index was in a range of 400 to 490 mmol/kg and 500 to 800 mmol/kg for the T2 and Oleozon ® oils, respectively. All the ozonized oils showed antimicrobial effect, finding that the higher activity was presented with a lower concentration of the inocula. The commercial oil has a significant difference (p‹0.05) respect to the T1 and T2 oils, having a higher effect. On the other hand, the T1 and T2 treatments did not show significant differences between them, whence the storage at this conditions did not affect the antimicrobial effect.


REFERENCES

  1. ALMEIDA NR, Beatriz A, Micheletti AC, Arruda EJ. 2013. Ozonized vegetable oils and therapeutic properties: A review. Orbital-The Electronic Journal of Chemistry. 4(4):313-26. ISSN 1984-6428, DOI: 10.17807/orbital.v4i4.467

  2. BRITISH Pharmacopoeia. 2000. Appendix XF, IA, IB. Peroxide value. http://www.uspbpep.com/bp2008/data/899.asp

  3. DÍAZ M, Lezcano I, Molerio J, Hernández F. 2001. Spectroscopic characterization of ozonides with biological activity. Ozone Science and Engineering. 23(1):35-40. ISSN: 1547-6545.DOI: 10.1080/01919510108961986

  4. DÍAZ MF, Gavín JA, Gómez M, Curtielles V, Hernández F. 2006. Study of ozonated sunflower oil using 1H NMR and microbiological analysis. Ozone: Science and Engineering. 28(1):59-63. ISSN: 1547-6545. DOI: 10.1080/01919510500479239

  5. DÍAZ MF, Gavín Sazatornil JA, Ledea O, Hernández F, Alaiz M, Garcés R. 2005. Spectroscopic characterization of ozonated sunflower oil. Ozone: Science and Engineering. 27(3):247-53. ISSN: 1547-6545. DOI: 10.1080/01919510590945822

  6. FERNÁNDEZ TI, Curtiellas Piñol V, Sánchez Urrutia E, Gómez Regueiferos M. 2006. In vitro antimicrobial activity of ozonized theobroma oil against Candida albicans. Ozone: Science and Engineering. 28(3):187-90. ISSN: 1547-6545. DOI: 10.1080/01919510600689380

  7. IORIO FB, Liberatore AM, Koh IH, Otani C, Camilo FF. 2016. Ozonated Mineral Oil: Preparation, Characterization and Evaluation of the Microbicidal Activity. Ozone: Science and Engineering. 38(4):253-60. ISSN: 1547-6545. DOI: 10.1080/01919512.2015.1128801

  8. KIM HS, Noh SU, Han YW, Kim KM, Kang H, Kim HO, Park YM. 2009. Therapeutic effects of topical application of ozone on acute cutaneous wound healing. Journal of Korean Medical Science. 24(3):368-74. ISSN: 1011-8934 DOI: 10.3346/jkms.2009.24.3.368

  9. MARTÍNEZ-SÁNCHEZ G, Re L, Davison GP, Delaporte RH. 2012. Las aplicaciones médicas de los aceites ozonizados: actualización. Revista Española de Ozonoterapia. 2(1):121-39. ISSN: 2174-3215

  10. MONTEVECCHi M, Dorigo A, Cricca M, Checchi L. 2013. Comparison of the antibacterial activity of an ozonated oil with chlorhexidine digluconate and povidone-iodine. A disk diffusion test. New Microbiologica. 36: 289-302. ISSN: 1121-7138

  11. MOUREU S, Violleau F, Ali Haimoud-Lekhal D, Calmon A. 2016. Influence of Storage Temperature on the Composition and the Antibacterial Activity of Ozonized Sunflower Oil. Ozone: Science and Engineering. 38(2):143-149. ISSN: 1547-6545. DOI: 10.1080/01919512.2015.1128319

  12. RAJABI O, Sazgarnia A, Abbasi F, Layegh P. 2015. The activity of ozonated olive oil against Leishmania major promastigotes. Iranian Journal of Basic Medical Sciences. 18(9):915. ISSN: 2008-3874. DOI: 10.22038/IJBMS.2015.5215

  13. SECHI LA, Lezcano I, Nunez N, Espim M, Duprè I, Pinna A, Molicotti P, Fadda G, Zanetti S. 2001. Antibacterial activity of ozonized sunflower oil (Oleozon). Journal of Applied Microbiology. 90(2):279-284. ISSN: 1365-2672. DOI: 10.1046/j.1365-2672.2001.01235.x

  14. SIFONTES AB, Marcos R, Avila EE, Villalobos-Duno HL. 2015. Uso clínico de los aceites ozonizados y su amplio espectro de las aplicaciones. Botica. 35:1-5. ISSN: 2443-4388

  15. SKALSKA K, Ledakowicz S, Perkowski J, Sencio B. 2009. Germicidal properties of ozonated sunflower oil. Ozone: Science and Engineering. 31(3):232-237. ISSN: 1547-6545. DOI: 10.1080/01919510902838669

  16. TRAVAGLI V, Zanardi I, Bocci V. 2009. Topical applications of ozone and ozonated oils as anti-infective agents: an insight into the patent claims. Recent Patents on Anti-infective Drug Discovery. 4(2):130-142. ISSN: 2212-4071. DOI: 10.2174/157489109788490271

  17. ALMEIDA NR, Beatriz A, Micheletti AC, Arruda EJ. 2013. Ozonized vegetable oils and therapeutic properties: A review. Orbital-The Electronic Journal of Chemistry. 4(4):313-26. ISSN 1984-6428, DOI: 10.17807/orbital.v4i4.467

  18. BRITISH Pharmacopoeia. 2000. Appendix XF, IA, IB. Peroxide value. http://www.uspbpep.com/bp2008/data/899.asp

  19. DÍAZ M, Lezcano I, Molerio J, Hernández F. 2001. Spectroscopic characterization of ozonides with biological activity. Ozone Science and Engineering. 23(1):35-40. ISSN: 1547-6545.DOI: 10.1080/01919510108961986

  20. DÍAZ MF, Gavín JA, Gómez M, Curtielles V, Hernández F. 2006. Study of ozonated sunflower oil using 1H NMR and microbiological analysis. Ozone: Science and Engineering. 28(1):59-63. ISSN: 1547-6545. DOI: 10.1080/01919510500479239

  21. DÍAZ MF, Gavín Sazatornil JA, Ledea O, Hernández F, Alaiz M, Garcés R. 2005. Spectroscopic characterization of ozonated sunflower oil. Ozone: Science and Engineering. 27(3):247-53. ISSN: 1547-6545. DOI: 10.1080/01919510590945822

  22. FERNÁNDEZ TI, Curtiellas Piñol V, Sánchez Urrutia E, Gómez Regueiferos M. 2006. In vitro antimicrobial activity of ozonized theobroma oil against Candida albicans. Ozone: Science and Engineering. 28(3):187-90. ISSN: 1547-6545. DOI: 10.1080/01919510600689380

  23. IORIO FB, Liberatore AM, Koh IH, Otani C, Camilo FF. 2016. Ozonated Mineral Oil: Preparation, Characterization and Evaluation of the Microbicidal Activity. Ozone: Science and Engineering. 38(4):253-60. ISSN: 1547-6545. DOI: 10.1080/01919512.2015.1128801

  24. KIM HS, Noh SU, Han YW, Kim KM, Kang H, Kim HO, Park YM. 2009. Therapeutic effects of topical application of ozone on acute cutaneous wound healing. Journal of Korean Medical Science. 24(3):368-74. ISSN: 1011-8934 DOI: 10.3346/jkms.2009.24.3.368

  25. MARTÍNEZ-SÁNCHEZ G, Re L, Davison GP, Delaporte RH. 2012. Las aplicaciones médicas de los aceites ozonizados: actualización. Revista Española de Ozonoterapia. 2(1):121-39. ISSN: 2174-3215

  26. MONTEVECCHi M, Dorigo A, Cricca M, Checchi L. 2013. Comparison of the antibacterial activity of an ozonated oil with chlorhexidine digluconate and povidone-iodine. A disk diffusion test. New Microbiologica. 36: 289-302. ISSN: 1121-7138

  27. MOUREU S, Violleau F, Ali Haimoud-Lekhal D, Calmon A. 2016. Influence of Storage Temperature on the Composition and the Antibacterial Activity of Ozonized Sunflower Oil. Ozone: Science and Engineering. 38(2):143-149. ISSN: 1547-6545. DOI: 10.1080/01919512.2015.1128319

  28. RAJABI O, Sazgarnia A, Abbasi F, Layegh P. 2015. The activity of ozonated olive oil against Leishmania major promastigotes. Iranian Journal of Basic Medical Sciences. 18(9):915. ISSN: 2008-3874. DOI: 10.22038/IJBMS.2015.5215

  29. SECHI LA, Lezcano I, Nunez N, Espim M, Duprè I, Pinna A, Molicotti P, Fadda G, Zanetti S. 2001. Antibacterial activity of ozonized sunflower oil (Oleozon). Journal of Applied Microbiology. 90(2):279-284. ISSN: 1365-2672. DOI: 10.1046/j.1365-2672.2001.01235.x

  30. SIFONTES AB, Marcos R, Avila EE, Villalobos-Duno HL. 2015. Uso clínico de los aceites ozonizados y su amplio espectro de las aplicaciones. Botica. 35:1-5. ISSN: 2443-4388

  31. SKALSKA K, Ledakowicz S, Perkowski J, Sencio B. 2009. Germicidal properties of ozonated sunflower oil. Ozone: Science and Engineering. 31(3):232-237. ISSN: 1547-6545. DOI: 10.1080/01919510902838669

  32. TRAVAGLI V, Zanardi I, Bocci V. 2009. Topical applications of ozone and ozonated oils as anti-infective agents: an insight into the patent claims. Recent Patents on Anti-infective Drug Discovery. 4(2):130-142. ISSN: 2212-4071. DOI: 10.2174/157489109788490271




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